Reliable identification technique for the analysis of a wide range of substances

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HPTLC is the High-Performance and automated form of Thin-Layer Chromatography (TLC). It provides much greater resolution and separation efficiency than TLC. It is reliable identification technique with wide acceptability.

It can generate chromatographic fingerprints that can be visualized using different light sources (visible light, UV 366 nm and UV 254 nm) and can be documented as electronic images for regulatory purposes.


Numerous features of HPTLC include:

  • High sample throughput
  • Short analysis time
  • Low costs per analysis
  • Simple operation
  • Lower solvent consumption which makes it a environmental friendly technique
  • Easy to understand
  • Highly reproducible
  • No prior treatment of solvents such as degasification
  • No interference from previous analysis due to the disposable stationary phase
  • Non-UV absorbing substances are detectable by post-chromatography derivatization


Due to disposable stationary phase, samples with biologically complex matrix can be analyzed which may lead to column clogging in chromatography techniques such as HPLC and GC-MS.

After separation, due to non-destructive nature of HPTLC, substances can be eluted and identified using NMR, IR, Mass Spectroscopy or another analytical apparatus.

HPTLC is frequently applied in quality control, process monitoring and research and development (R&D) laboratories for daily analysis.

HPTLC is commonly employed to analyse a variety of substances such as alcohols, hydrocarbons, phenols, vitamins, cyclophosphamide, antibiotics, carbohydrates, organic acids, lipids, steroids, amino acids, pesticides, proteins, alkaloids, enzymes, pesticides, nucleic acids, organic sulphur and phosphorus, peptides, herbal drugs, organometallic compounds, poisons, dyes as well as plastics.

In addition, HPTLC is valuable technique for the separation of the chiral compounds.

biostep GmbH provides you the most professional TLC/HPTLC products to identify your compounds.