Identification and Characterization of Natural dyes Maddar and Indigo

Identification and Characterization of Natural dyes Maddar and Indigo
In this communication, an attempt has been made to compare the two major classes of natural dyes Anthraquinoid and Indigoid with their synthetic analogs. Maddar (Rubia cordifolia and Rubia tinctoria) and Indigo (Indigofera tinctoria) dyes were used for this study. HPTLC imaging system ProViDoc DD 70 and densitometer CD60 (scanner) were used to identify the purity content. The techniques are shown for providing a high degree of chemical information making dye identification highly specific. Taking images of the dye eluted plate by imaging system ProViDoc DD70 and developing chromatogram of the same by the densitometer CD60 with a light beam of visible light and UV ranges of 254 and 366 nm yielded very distinctive features. Fluorescence was measured and recorded.
