Detection and Determination of Caffeine in Caffeine Shampoos

Caffeine-containing products have been consumed for hundreds of years for their pleasant flavor and stimulating effects on central nervous system. Hair care products containing caffeine targeted to stimulate hair growth and reduce hair loss [1]. High-Performance Thin-Layer Chromatography (HPTLC) is a useful analytical technique which requires low sample preparation. Different samples can be analyzed very fast and simultaneously under the same conditions. Here qualification of the caffeine in caffeine shampoos from different suppliers was detected under UV light (254 nm) and the quantification was carried out by densitometry.

Application Note No.12